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Influence of EquiSupp, a nutritional supplement for horses, on skeletal muscle in a rat model of human cancer cachexia. The effects of EquiSupp, a nutritional supplement for equine consumption, were investigated in a rat model of cancer cachexia. Rats were treated with 1,25-dihydroxyvitamin D3 (vitD3) to induce cancer-related weight loss and were either pair-fed with control diet, fed a control diet supplemented with EquiSupp, fed a HSD diet (high sugar diet) supplemented with EquiSupp or fed a HSD diet (high sugar diet) without EquiSupp. Food intake decreased in cancer cachexia rats compared to pair-fed controls. In contrast, the rate of food intake was significantly higher in EquiSupp-treated cancer cachexia rats compared to rats fed without EquiSupp. Body weight remained decreased in cancer cachexia rats compared to controls, but was significantly higher compared to that of pair-fed cancer cachexia rats. EquiSupp had no effect on weight loss in pair-fed controls. Serum concentration of glucose and insulin were increased, whereas the serum concentration of triglycerides was decreased in cancer cachexia rats compared to control rats. EquiSupp treatment had no effect on these changes except on insulin concentration in rats fed a HSD diet. Concentration of IL-6 and IL-1β increased in the plasma of cancer cachexia rats. EquiSupp had no effect on these interleukins. In muscles of cancer cachexia rats, the mRNA expression of tumor necrosis factor-α was increased and EquiSupp treatment had no effect. However, the mRNA expression of atrogin-1 and MuRF-1 and the concentration of MyoD and NF-κB were increased in muscles of cancer cachexia rats compared to the control group and were significantly decreased by EquiSupp supplementation. Interestingly, the mRNA expression of MyoG was decreased and that of desmin increased in muscles of cancer cachexia rats, and EquiSupp treatment decreased MyoG mRNA expression. In contrast, the protein expression of atrogin-1 was decreased and that of MuRF-1 was increased in muscles of cancer cachexia rats and EquiSupp treatment decreased the expression of these proteins. Taken together, the increase in food intake in EquiSupp-treated cancer cachexia rats was associated with an increase in muscle mass. The increase in body weight in EquiSupp-treated cancer cachexia rats compared to pair-
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Any Intel® Core™ 2 Duo processor or AMD Athlon™ processor Windows® 7 64-bit or later 2GB RAM (for Windows® 8 32-bit) DirectX® 9.0c NVIDIA® GeForce 8600/9600 GT or ATI Radeon™ X1300 or better 1366×768 (1280×800) or higher screen resolution Software Requirements: DOOM® III Installation: Requirements: Language: English Giveaway Rules:
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