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nitro pro 7.3.7 serial numberate (Sigma) and grown in shaking cultures at 37 °C, 250 rpm to OD~600~ ∼ 0.7. Cultures were then diluted to OD~600~ ∼ 0.1 and grown for an additional 20 min at 37 °C, 250 rpm. For every experiment, two biological replicates were run, and each sample was independently prepared and analysed in triplicate. Flow cytometry {#Sec12} ————– Flow cytometry was performed on BD LSRII or BD Canto II instruments. Flow cytometric settings for cell counting and cell sorting were used to detect cell size and DNA content per cell, with and without the addition of propidium iodide (1 µg/ml) as needed to distinguish nuclei and nuclei from dead cells. For each sample, a minimum of 10,000 events were acquired. Flow cytometry data was analysed using FlowJo software (FlowJo, LLC). In the case of Propidium iodide (PI) quantitation, each sample was gated for single cells according to forward and side scattering characteristics, and nuclei were selected by compensation settings within the PI detector channel. For each sample, the nuclear PI signal intensity for both bright and dim events was plotted against the total PI fluorescence intensity for all events; a linear function was fitted to the data, and the initial concentration of PI was estimated from this slope. In all cases, statistical comparisons were performed using two-tailed Student’s *t*-test. Transcription reporter assays {#Sec13} —————————– *E. coli* strain BW25113 carrying plasmid pWQQ72 (kindly provided by Prof. Wei Gu, Colorado State University) and plasmid pCUMS.K72^[@CR65]^ were transformed with pYH27. The cells were grown to OD~600~ 0.6, diluted to OD~600~ 0.05 and 1 µg/ml D-lactate was added for 3 h to induce CUT expression. From each culture, 100 µl was collected from three separate independent cultures and genomic DNA was isolated using the protocol described above. Recipient WT *E. coli* strain MG1655 and mutants were transformed with DNA-free plasmids pWQQ72 and pCU