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. The sham-operated control group underwent a sham-operation and received the same amount of liquid as in the other treatment groups. The animals were anesthetized via an injection of ketamine (100 mg/kg). The abdomen was opened through an incision made in the linea alba, and the uterine horns were exposed. The uterine horns were raised off of the abdominal muscles, and the mucosal layer was carefully incised and retracted to expose the serosa layer. The uterine horns were tied using a loose knot, and the incision was closed using a suture.

Tissue preparation for histopathology {#Sec13}
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After 6 weeks of treatment, the animals were sacrificed via an overdose of anesthetic. The uteri were removed, and the adnexa were removed. Tissues were fixed in 10% formalin and embedded in paraffin. The paraffin-embedded tissues were sectioned, and the sections were stained with hematoxylin and eosin. The tissues were processed and stained using standard procedures. A light microscope (OLYMPUS IX70; Olympus Corp., Tokyo, Japan) was used for analysis of the sections.

Apoptosis assessment by terminal deoxynucleotidyl transferase dUTP nick end labeling assay {#Sec14}
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Apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The TUNEL assay was performed using the DeadEnd™ Colorimetric TUNEL System (Promega, Madison, WI, USA). Tissues were fixed in 4% neutral buffered formalin for 24 h. Tissues were then processed for paraffin embedding and sectioned at 4 μm thickness. The sections were de-paraffinized and permeabilized using standard methods. The sections were incubated with proteinase K for 20 min. Next, the sections were washed, and a labeled reaction mixture was added. The mixture was incubated for 60 min. After

 

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